Lab Skills: Learn Spectrophotometry
Date(s) - Saturday, March 30, 2019
2:00 pm - 6:00 pm
Biotech Without Borders
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About lab skills workshops:
In this ongoing series of 1-2 day courses at Biotech without Borders, we’re inviting people of all skill levels together to complete a small teaching exercise in order to learn about laboratory work. We will cover the proper use of equipment and the real world application of the techniques being practiced.
Learn Spectrophotometry: Measure the Concentration of a Molecule
How does a scientist know the concentration of molecule in solution? One of the methods commonly used in the molecular biology laboratory is spectrophotometry. This technique passes light through a sample and measures how much is absorbed to calculate the concentration. In this workshop, you will prepare your own standard curve in cuvettes to calibrate a spectrophotometer. Then you will measure the concentration of an unknown sample. Spectrophotometry is a workhorse laboratory technique and the basis of innumerable biological and chemical assays. Participants will get hands-on experience using micropipettes, an analytical balance, a spectrophotometer and participate in graphing your results in R.
About the insturctor:
nstructor Danny Chan received his MSc in microbiology during the course of his PhD candidacy at the University of Chicago studying the interaction of methicillin resistant staphylococcus aureus (MRSA) with lab-grown skin in an a department focused on infectious disease. He has been a research technician for many years in multiple fields including cellulostic ethanol production, protein crystallography, prefrontal cortex development and heat shock proteins. He seeks to apply his skills and knowledge of science in order to foster new institutions of research and learning. Currently, he makes his living fact checking pharmaceutical ads in an agency while trying to defend his time to pursue independent research centered around protocol development for the DIY science community and teaching. You can follow his activities online HERE